De isomer bicyclo[4.1.0]heptane analogue galacto-validamine inhibiteur glikozidaz la, (1R*,2S,3S,4S,5S,6S*)-5-amino-1-(hydroxymethyl)bicyclo[4.1.0]heptane. -2,3,4-triol, yo te sentèz nan 13 etap soti nan 2,3,4,6-tetra-O-benzyl-D-galaktoz.Aktivite inhibitor de amine ki gen restriksyon nan konformasyon yo, ak asetamid korespondan yo, yo te mezire kont anzim alfa-galactosidase komèsyal ki soti nan pwa kafe ak E. coli.Aktivite glycosyl hydrolase fanmi GH27 anzim (pwa kafe) te konpetitif inibit pa 1R,6S-amine (7), yon entèraksyon Liaison ki te karakterize pa yon valè K(i) de 0.541 microM.GH36 E. coli alfa-galactosidase a te montre yon pi fèb entèraksyon Liaison ak 1R,6S-amine (IC(50)= 80 microM).1S,6R-amine diastereomeric (9) mare fèb nan tou de galactosidases, (pwa kafe, IC(50)= 286 microM) ak (E. coli, IC(50)= 2.46 mM).
